Multiplexing of ChIP-Seq Samples in an Optimized Experimental Condition Has Minimal Impact on Peak Detection
نویسندگان
چکیده
منابع مشابه
Multiplexing of ChIP-Seq Samples in an Optimized Experimental Condition Has Minimal Impact on Peak Detection
Multiplexing samples in sequencing experiments is a common approach to maximize information yield while minimizing cost. In most cases the number of samples that are multiplexed is determined by financial consideration or experimental convenience, with limited understanding on the effects on the experimental results. Here we set to examine the impact of multiplexing ChIP-seq experiments on the ...
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ChIP-SEQ AND PEAK CALLING
et al details each of these steps and discusses how peak finding tools approach the separate steps very differently (3). A follow up review by Wilbanks et al evaluated the performance of 11 ChIP-seq peak callers nearly all of which are still widely used today (4). Each step can have parameters that can be adjusted by the user, but changing these can significantly affect the final peak lists. Ca...
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Motivation and Objectives ChIP-Seq has rapidly become the dominant experimental technique in functional genomic and epigenomic research. Statistical analysis of ChIP-Seq data sets however remains challenging, due to the highly structured nature of the data and the paucity of replicates. Current approaches to detect differentially bound or modified genomic regions are mainly borrowed from RNA-Se...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2015
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0129350